qPCR Data Analysis


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Documentation for package ‘rtpcr’ version 2.1.0

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ANOVA_DCt Relative expression analysis using the Delta C_T method with ANOVA
ANOVA_DDCt Relative expression analysis using the DeltaDelta C_T method with ANOVA and ANCOVA
efficiency Amplification efficiency statistics and standard curves
Means_DDCt Relative expression (Delta Delta C_T) analysis using a fitted model
meanTech Calculate mean of technical replicates
multiplot Combine multiple ggplot objects into a single layout
plotOneFactor Bar plot of gene expression for single-factor experiments
plotThreeFactor Bar plot of gene expression for three-factor experiments
plotTwoFactor Bar plot of gene expression for two-factor experiments
REPEATED_DDCt Fold change (DeltaDelta C_T) analysis of repeated-measure qPCR data
TTEST_DDCt Expression analysis of target genes using the DeltaDelta C_T method and t-test